Biopsies
General Principles
Checklist and Protocols - Pre-Procedural, Procedural and Post-Procedural
Pre-Procedural
- Review of the case and choosing the right lesion to biopsy
 - Coagulation check and tranexamic acid prophylaxis
 - Consent
 
Procedural
- FNAC vs Biopsy
 - Machine
 - Tray
 - Guns
 - Sedation and Anesthesia
- General / Conscious
 - Local - pleural, diaphragmatic, traversing nerves
 
 - Sampling (how much and for what)
- Case 68: 26-years old - L3 body lesion - transpedicular approach and perivertebral soft tissue - how to maximize obtaining adequate material - tuberculosis
 - Case 85: 66-years old - L4/5 discitis - posterolateral perivertebral approach - post-operative spine - don't forget fungal - Candida fungal infection
 - Case 86: 26-years old - L5/S1 infectious spondylitis - S1 ala biopsy - don't forget fungal (geography matters) - Coccidiodes immitis infection
 
 - Bone and Soft Tissue Mass Biopsy Rules
 - Diagnosis on Table
- Case 49: 75-years old - L4 body lesion - transpedicular approach - black tissue cores - melanoma metastasis
 - Case 50: 34-years old - pretracheal and paratracheal mass - extrapleural approach with hydrodissection - rock hard to feel - mediastinal fibrosis
 - Case 51: 32-years old - apical posterior mediastinal mass - electric shock on touching - schwannoma
 - Case 57: 40-years old - left adrenal nodule - posterior approach - skin lesions of Addison's disease - histoplasmosis
 - Case 147: 54-years old - mesenteric mass - anterior approach - milky white lymph aspirate - mesenteric lymphatic cyst
 
 - Tips and Tricks
- Gantry Angulation
 - Patient Positioning
- Flexion/Extension
 - Changing Positions
 - Decubitus (see lung nodules)
 
 - Needle Angulation
 - Understanding the Importance of Stylet Length During Needle Placement
 - Flipping / Multidirectional / Multiquadrant biopsy
 - Railroading
 - Managing Mismatch Between Lesion Length in Bone Versus Tray and Tip Length
 - Correcting Breathed Out Needles
 - Transorgan
- Transosseous
- Case 36: 36-years old - prevascular space masss - transsternal approach - germ cell tumor - seminoma
 - Case 37: 53-years old - prevascular space mass - transsternal transpulmonary approach - thymoma
 - Case 38: 76-years old - pretracheal space mass - transsternal approach - adenocarcinoma
 - Case 39: 23-years old - loculated pleural collection - transscapular approach - tuberculosis
 - Case 62: 60-years old - right common iliac node - transsacral approach - metastasis
 - Case 84: 20-years old - sacroilitis - prone approach - transosseous - transiliac bone - tuberculosis
 - Case 156: 72-years old - tuberculosis - left para-aortic nodal mass - transsternal approach
 
 - Transpulmonary
 - Transpleural (pneumothorax, effusion, collection)
 - Transhepatic
 - Transpancreatic
 - Transbowel
 - Transiliopsoas
 - Transthyroid
 
 - Transosseous
 - Displacing Structures (Bowel, Organs, etc)
 - Fixed Point Method
 - Blind Biopsies - Using Measurements and Feel
 - Curved/Bent Needle Technique
 - Blunt-Tip Needle Technique to Bypass Bowel, Vessels, etc
- Case 151: 55-years old - pancreatic body lesion biopsy – using a blunt-tip stylet to bypass stomach - adenocarcinoma
 - Case 153: 47-years old - pancreatic head lesion biopsy – using a blunt-tip stylet to bypass vessels with an almost 0 mm narrow window - adenocarcinoma
 - Case 164: 69-years old - old Ca ovary/endometrium with new pelvic mesenteric nodes - blunt-tip to part the bowel loops - metastatic adenocarcinoma
 
 - Intraprocedural Contrast (See also Pancreas, Porta, Mediastinum)
 - Hydrodissection
- Case 1: 12-years old - right paratracheal node biopsy with an extrapleural approach
 - Case 30: 15-years old - 31 mm subcarinal node - extrapleural approach - tuberculosis
 - Case 45: 17-years old - 21.1 mm subcarinal node - extrapleural - tuberculosis
 - Case 77: 54-years old - left para-aortic mass - parasternal approach with hydrodissection (salinoma) - metastasis
 
 - Sclerotic Bone Biopsy
 
 - Complications
- Do No Harm
 - Bleeding
 - Pneumothorax (see in lung)
- Case 8: Respect the lung
 - Case 18: Managing entry pneumothorax
 - Entry and exit pneumothorax - strategies to reduce incidence and manage if the pneumothorax still occurs
 - Case 99: 69-years old - lung mass - pneumothorax, aspirated
 - Case 122: 64-years old - lung adenocarcinoma - rapid rollover to reduce the rate of pneumothorax and chest drain insertion
 
 - Vasovagal
 
 
Post-Procedural
- Managing samples
 - Managing patients
 - Follow-up of reports and patients
- Case 45: 17-years old - 21.1 mm subcarinal node - extrapleural - the need for patience - all initial reports negative - only culture at 6 weeks positive
 - Case 46: 75-years old - D12 vertebral body tuberculosis - costovertebral approach - the need for patience - all initial reports negative - only culture positive at 6 weeks.
 - Case 65 - 22-years old - sacrolitis - prone approach - the value of patience and waiting for the culture report before repeating a biopsy - tuberculosis
 
 - Repeating biopsies
 
Individual Organs
Chest
- 
Lung
- Masses (> 30 mm)
 - Nodules
- 
Solid
- < 6 mm
 - 6-10 mm
 - 10-20 mm
- Case 17: 62-years old - 15 mm nodule right lower lobe - metastasis
 - Case 34: 34-years old - 18 mm right middle lobe subpleural nodule - left lateral decubitus - metastasis
 - Case 35: 63-years old - 10.4 mm right lower lobe nodule - spearing the nodule - tuberculosis
 - Case 76: 57-years old - 15.2 mm right upper lobe lung nodule - multiple positioning attempts - supine oblique - needle breathed out - metastasis
 - Case 120: 67-years old ex-smoker with emphysema - 16.7 mm right upper lobe lung nodule - adenosquamous carcinoma - discussion on when to fly back
 - Case 127: 55-years old - 15.5 mm cavitated lung nodule - biopsy of superior wall - metastasis from ampulla of Vater carcinoma
 
 - 20-30 mm
- Case 15: 75-years old - 24 mm nodule right lower lobe with IPF - prone - squamous cell ca
 - Case 67: 66-years old - 23 mm nodule right middle lobe - left lateral decubitus - tuberculosis
 - Case 81: 60-years old - 20.22 mm left apical nodule - gantry angulation - organizing pneumonia
 - Case 122: 64-years old - 21.9 mm known lung adenocarcinoma for mutation studies and rapid rollover to reduce the rate of pneumothorax and chest drain insertion
 - Case 146: 73-years old - 20.5 mm lung nodule with UIP-IPF - granulomatous
 
 
 - 
Subsolid
 
 - 
 - Consolidation
 
 - 
Mediastinum
- Prevascular Space
- Case 36: 36-years old - prevascular space masss - transsternal approach - germ cell tumor - seminoma
 - Case 37: 53-years old - prevascular space mass - transsternal transpulmonary approach - thymoma
 - Case 69: 30-years old - prevascular space mass - parasternal approach - lymphoma
 - Case 137: 46-years old - prevascular space mass - right costoclavicular approach - lymphoma
 - Case 141: 56-years old - thymoma - thymic tumor biopsies, seeding issues, etc
 - Case 156: 72-years old - tuberculosis - left para-aortic nodal mass - transsternal approach
 - Case 158: 51-years old - buccal carcinoma with biopsy of thyroid lesion and prevascular space precaval mediastinal node in the same sitting - both squamous cell carcinoma metastases
 
 - Left Para-aortic and Aortopulmonary Window
 - Paratracheal / Pretracheal (Visceral Space)
- Case 1: 12-years old - right paratracheal node biopsy with an extrapleural approach - tuberculosis
 - Case 38: 76-years old - pretracheal space mass - transsternal approach - adenocarcinoma
 - Case 50: 34-years old - pretracheal and paratracheal mass - extrapleural approach with hydrodissection - mediastinal fibrosis
 
 - Subcarinal
- Case 30: 15-years old - 31 mm subcarinal node - extrapleural approach with hydrodissection (salinoma) - tuberculosis
 - Case 31: 36-years old - 20 mm subcarinal node - transpulmonary approach - tuberculosis
 - Case 32: 55-years old - 26.4 mm subcarinal node - transpleural approach - pre-existing pneumothorax - sarcoidosis
 - Case 45: 17-years old - 21.1 mm subcarinal node - extrapleural approach with hydrodissection (salinoma) - tuberculosis
 
 - Posterior Mediastinum (Paravertebral & Others)
 - Others
 
 - Prevascular Space
 - 
Pleura
- Case 39: 23-years old - loculated pleural collection - transscapular approach - tuberculosis
 - Case 106: 71-years old - pleural thickening - metastastic adenocarcinoma
 - Case 114: 41-years old - small dry pleural nodule - metastatic thymoma
 - Case 130: 71-years old - small pleural nodule - metastasis from lung adenocarcinoma - parallel approach
 - Case 143: 71-years old - small visceral pleural nodule - metastasis from carcinoma tongue - long parallel approach from the opposite side
 
 
Abdomen and Pelvis
- 
Liver
- Case 97: 50-years old - multiple liver lesions & pancreatic body lesion - segment 4B liver mass biopsy - metastasis from pancreatic primary
 - Case 102 - 42-years old - isodense liver lesion not seen on USG - measurements and feel - metastasis from carcinoma breast
 - Case 145: 66-years old - caudate lobe surface lesion - transpancreatic route - metastatic renal cell carcinoma
 
 - 
Gallbladder
- Case 100: 46-years old - gallbladder mass infiltrating liver - high grade undifferentiated carcinoma
 - Case 101: 46-years old - gallbladder wall mass with eccentric nodular thickening - small cell carcinoma
 - Case 163: 56-years old - gallbladder mass - fundus with liver involvement - refused by USG experts
 
 - 
Porta
 - 
Pancreas
- Head & Uncinate Process
- Case 6: 64-years old - uncinate process & head mass - adenocarcinoma
 - Case 79: 57-years old - pancreatic head mass - transcolonic approach - adenocarcinoma
 - Case 82: 66-years old - pancreatic head mass - displacing bowel - adenocarcinoma
 - Case 112: 63-years old - diffuse pancreatic disease - IgG4 disease
 - Case 153: 47-years old - pancreatic head lesion biopsy – using a blunt-tip stylet to bypass vessels with an almost 0 mm narrow window - adenocarcinoma
 
 - Body
 - Tail
 
 - Head & Uncinate Process
 - 
Spleen
 - 
Adrenal Glands
- Case 21: 76-years old - 11.6 mm left adrenal nodule - ipsilateral decubitus - tuberculosis
 - Case 22: 70-years old - 18.8 mm left adrenal nodule - posterolateral approach - metastasis
 - Case 56: 47-years old - 11.3 mm left adrenal nodule - posterior approach - tuberculosis
 - Case 57: 40-years old - 23 mm left adrenal nodule - posterior approach - histoplasmosis
 - Case 90: 58-years old - 18.4 mm left adrenal nodule - posterior approach with bending of the needle for a curve needle approach - metastasis
 - Case 150: 40-years old - >30 mm right adrenal gland nodule - posterior, mediolateral approach - pheochromocytoma
 
 - 
Kidneys
 - 
Ureters
 - 
Bowel
 - 
Omentum and Peritoneum
 - 
Mesentery
- Case 80: 64-years old - mesenteric mass - transcolonic approach - metastasis
 - Case 147: 54-years old - mesenteric mass - anterior approach - milky white lymph aspirate - mesenteric lymphatic cyst
 - Case 164: 69-years old - old Ca ovary/endometrium with new pelvic mesenteric nodes - blunt-tip to part the bowel loops - metastatic adenocarcinoma
 
 - 
Retroperitoneum
- Retrocrural
 - Para-aortic
- Case 42: 38-years old - complex retroperitoneal mass - prone - para-aortic biopy with intravenous contrast - pancreatic adenocarcinoma
 - Case 43: 64-years old - left para-aortic node from carcinoma sigmoid - first prone, then supine - for mutation studies
 - Case 93: 86-years old - left para-aortic node biopsy - metastasis from endometrial carcinoma - multidirectional rotation of the biopsy gun
 - Case of the Day 106 - 55-yrs old - Retroperitoneal posterior parapsoas approach - macrocystic lymphatic malformation aspiration
 
 - Aortocaval
 - Retrocaval / paracaval
 - Pre-Aortocaval
 - Periaortic
 
 - 
Pelvis
- Ovary
 - Uterus
 - Nodes and Nodules
- Case 62: 60-years old - right common iliac node - transsacral approach - metastasis
 - Case 63: 41-years old - left iliac bifurcation node - anterior transperitoneal approach - necrosis, no viable tumor
 - Case 88: 60-years old - right iliac bifurcation node - transiliopsoas approach - metastasis
 - Case 164: 69-years old - old Ca ovary/endometrium with new pelvic mesenteric nodes - blunt-tip to part the bowel loops - metastatic adenocarcinoma
 - Case 169: 66-years old - right common iliac node - posterior transiliotransverse ligament approach - metastatic breast carcinoma
 
 
 
Axial Skeleton
- 
Skull
- Case 58: 59-years old - left frontal skull bone osteolytic lesion - metastasis
 - Case 59: 29-years old - left posterior parietal bone osteolytic lesion - brown tumor
 - Case 161: 59-years old - left squamous temporal bone lesion - metastasis
 - Case 162: 70-years old - left frontal subgaleal calvarial soft tissue lesion - non-Hodgkin's lymphoma
 
 - 
Spine
- Lecture: Approach to Spine Biopsies - Tips and Tricks
 - Lecture: The Role of Image Guided Biopsy - When the Biopsy Makes a World of Difference
 - Snippet: Why Every Infectious Spondylitis Needs a Biopsy - Three Recent Unusual Spine Infections
 - Craniovertebral Junction
 - Cervical
- C2-C5
 - C5-C7
- Case 25: 41-years old - C6/7 infective disciitis - transthyroid approach - Streptococcus agalactiae
 - Case 26: 50-years old - C6/7 infective disciits - transthyroid approach - E.coli
 - Case 167: 60-years old - metastatic adenocarcinoma from breast - transpedicular approach for a C7 body lesion
 - Case of the Day 098: 16-years old - tuberculosis C6 vertebra - anterolateral retrocarotid approach
 
 
 - Dorsal
- Case 11: 16-years old - D3 transverse process lesion - Langerhans cell histiocytosis
 - Case 46: 75-years old - D12 body lesion - costovertebral approach - tuberculosis
 - Case 73: 59-years old - D2 body lesion - costovertebral approach - lymphoma
 - Case 131: 48-years old - D2/3 infectious spondylitis - transpedicular approach - Brucellosis
 - Case of the Day 100: 37-years old - D1 giant cell tumor - transpedicular approach
 
 - Lumbar
- Case 3: 40-years old - L2 vertebral lesion - perivertebral biopsy and psoas aspiration - tuberculosis
 - Case 49: 75-years old - L4 body lesion - transpedicular approach - melanoma metastasis
 - Case 68: 26-years old - L3 body lesion - transpedicular approach and perivertebral soft tissue - tuberculosis
 - Case 75: 49-years old - L5/S1 discitis - transsacral posterolateral approach - no specific diagnosis
 - Case 85: 66-years old - L4/5 discitis - posterolateral perivertebral approach - post-operative spine - Candida fungal infection
 - Case 165: 64-years old - L5 osteolytic lesion - transfacet joint approach - tuberculosis
 
 - Sacrum
 
 - 
Sacroiliac (SI) Joints
- Case 60: 63-years old - sacroilitis - prone approach - MRSA Staphylococcus aureus infection
 - Case 61: 29-years old - sacroilitis - prone approach - tuberculosis
 - Case 65: 22-years old - sacroilitis - prone approach - tuberculosis
 - Case 84: 20-years old - sacroilitis - prone approach - transosseous - transiliac bone - tuberculosis
 
 - 
Chest Wall
- Axilla
 - Ribs
 - Internal Mammary
 - Intercostal Space
 - Costal Cartilage & Costochondral Junction
 - Sternum
 - Scapula
 - Clavicle
 
 - 
Bony Pelvis
- Case 10: 26-years old - right ischial tuberosity biopsy - Langerhans cell histioytosis (LCH)
 - Case 12: 74-years old - multifocal osteolytic and osteosclerotic bone lesions - right iliac bone osteolytic lesion - non-Hodgkin's lymphoma
 - Case 64: 49-years old - multifocal osteolytic and osteosclerotic bone lesions - repeat biopsy done from osteolytic and sclerotic foci - three sites - lymphoma
 - Case 94: 47-years old - iliopubic ramus biopsy using railroading - squamous cell metastasis from buccal carcinoma
 - Case 144: 77-years old - ischiopubic ramus biopsy of a focal sclerotic lesion - metastastic adenocarcinoma from prostate
 
 
Appendicular Skeleton
- 
Lecture
 - 
Bones & Joints
- Upper Extremity
 - Lower Extremity
- Case 66: 60-years old - distal femur lesion - clear cell chondrosarcoma
 - Case 121: 28-years old - transtrochanteric biopsy of femoral neck lesion - Langerhans cell histioyctosis
 - Case 152: 62-years old - hip joint biopsy - tuberculosis
 - Case 160: 22-years old - mid femur diaphyseal lesion biopsy - Salmonella typhi
Case of the Day 094: 13-years old - proximal tibial osteosarcoma - hard bone - biopsy through Sharpey's fibres 
 
 - 
Soft Tissues
- Upper Extremity
 - Lower Extremity
 
 
Head & Neck
- 
Suprahyoid
- Case 23: 23-years old - sphenoid bone lesion - subzygomatic approach - adenoid cystic carcinoma
 - Case 24: 66-years old - oropharygeal mass - paramaxillary approach - non-Hodgkin's lymphoma
 - Case 78: 47-years old - lateral pharyngeal/retropharyngeal mass - paramaxillary approach - metastasis
 - Case 113: 60-years old - intra-maxillary sinus biopsy - anterolateral approach - lymphoma
 - Case 123: 78-years old - nasopharyngeal mass - paramaxillary approach - inflammation
 - Case 133: 81-years old - deep parotid lesion biopsy - retromandibular, premastoid approach - oncocytoma
 - Case 149: 54-years old - upper buccal space - paramaxillary approach - squamous cell carcinoma recurrence
 
 - 
Infrahyoid
 
Ablations
Radiofrequency Ablation (RFA)
- 
Bone
- Osteoid Osteoma
 - Chondroblastoma
 - Osteoblastoma
 - Metastases
 
 - 
Lung
 - 
Liver
 
Cryoablation
- 
Liver
 - 
Renal Tumors
 - 
Fibromatosis
 - 
Bone Tumors
 
Tumor Injection Ablations
- 
Aneurysmal Bone Cyst
 - 
Langerhans Cell Histiocystosis
 
